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Although the 3D structures of active and inactive cannabinoid receptors type 2 (CB2) are available, neither the X-ray crystal nor the cryo-EM structure of CB2-orthosteric ligand-modulator has been resolved, prohibiting the drug discovery and development of CB2 allosteric modulators (AMs). In the present work, we mainly focused on investigating the potential allosteric binding site(s) of CB2. We applied different algorithms or tools to predict the potential allosteric binding sites of CB2 with the existing agonists. Seven potential allosteric sites can be observed for either CB2-CP55940 or CB2-WIN 55,212-2 complex, among which sites B, C, G and K are supported by the reported 3D structures of Class A GPCRs coupled with AMs. Applying our novel algorithm toolset-MCCS, we docked three known AMs of CB2 including Ec2la (C-2), trans-β-caryophyllene (TBC) and cannabidiol (CBD) to each site for further comparisons and quantified the potential binding residues in each allosteric binding site. Sequentially, we selected the most promising binding pose of C-2 in five allosteric sites to conduct the molecular dynamics (MD) simulations. Based on the results of docking studies and MD simulations, we suggest that site H is the most promising allosteric binding site. We plan to conduct bio-assay validations in the future. 

The Unmanned aerial vehicles (UAVs) sector is fast-expanding. Protection of real-time UAV applications against malicious attacks has become an urgent problem that needs to be solved. Denial-of-service (DoS) attack aims to exhaust system resources and cause important tasks to miss deadlines. DoS attack may be one of the common problems of UAV systems, due to its simple implementation. In this paper, we present a software framework that offers DoS attack-resilient control for real-time UAV systems using containers: Container Drone. The framework provides defense mechanisms for three critical system resources: CPU, memory, and communication channel. We restrict the attacker's access to the CPU core set and utilization. Memory bandwidth throttling limits the attacker's memory usage. By simulating sensors and drivers in the container, a security monitor constantly checks DoS attacks over communication channels. Upon the detection of a security rule violation, the framework switches to the safety controller to mitigate the attack. We implemented a prototype quadcopter with commercially off-the-shelf (COTS) hardware and open-source software. Our experimental results demonstrated the effectiveness of the proposed framework defending against various DoS attacks.